Molecular Ecology and Health (MECOH) lab - Ehime Uni., Japan / 愛媛大学 分子生態・保健研究室( 渡辺幸三研究室)
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Sample collection from pig farm wastewater

7/21/2022

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The AMR team (Prof. Kozo Watanabe, Yuichiro Shimada, Ryota Ueda, Ngure Kagia, and Kenneth Bongulto) collected wastewater samples from a pig farm  last July 14, 2022. The aim of the sample collection is to isolate environmental bacteria and test for antimicrobial resistance genes (ARGs).
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Wastewater samples were collected from three different sections (influent, sequencing batch reactor, and effluent basin) of the wastewater treatment system. Environmental parameters were also gathered per sampling site.
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Second eDNA sampling in Satsunai river, Hokkaido, Japan

7/12/2022

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Environmental DNA (eDNA) sampling was performed again in Satsunai river, Hokkaido, Japan last July 6 to 10. Water samples for eDNA detection were collected from river and hyporheic zone. Macroinvertebrates both from benthic and hyporheic zones were also collected for morphological identification and DNA metabarcoding analysis.
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Professor Negishi from Hokkaido University performs water sampling from the hyporheic zone of Satsunai river for eDNA detection of macroinvertebrates.
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Water sample collected from the hyporheic zone were aseptically transferred to a sterile container  for transport.
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Dan a Research student from MECOH lab performs water collection from the Satsunai river for eDNA detection.
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Dan together with the graduate students from Hokkaido University.
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