The AMR team (Prof. Kozo Watanabe, Yuichiro Shimada, Ryota Ueda, Ngure Kagia, and Kenneth Bongulto) collected wastewater samples from a pig farm last July 14, 2022. The aim of the sample collection is to isolate environmental bacteria and test for antimicrobial resistance genes (ARGs).
Wastewater samples were collected from three different sections (influent, sequencing batch reactor, and effluent basin) of the wastewater treatment system. Environmental parameters were also gathered per sampling site.
Environmental DNA (eDNA) sampling was performed again in Satsunai river, Hokkaido, Japan last July 6 to 10. Water samples for eDNA detection were collected from river and hyporheic zone. Macroinvertebrates both from benthic and hyporheic zones were also collected for morphological identification and DNA metabarcoding analysis.
Professor Negishi from Hokkaido University performs water sampling from the hyporheic zone of Satsunai river for eDNA detection of macroinvertebrates.
Water sample collected from the hyporheic zone were aseptically transferred to a sterile container for transport.
Dan a Research student from MECOH lab performs water collection from the Satsunai river for eDNA detection.
Dan together with the graduate students from Hokkaido University.